Cells were seeded on a petri dish overnight to encourage adhesion, and then, 2 μl of the membrane fluorescent probe DiO (initial concentration, 1000 μM) was added. After a 20-min GO incubation (10 μg/ml), cells were immediately monitored by an UltraVIEW (Perkin Elmer) under a 100× oil objective. Regions of interest in the stained membranes were noted and photobleached using a 488-nm laser operating at 13% power. Single images were then collected at maximum speed. All FRAP data were analyzed with the prepackaged analysis software, which can fit the model to experimental data reasonably through a nonlinear curve fit to f(t) = y + Aekt.

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