The total release of cytoplasmic lactate dehydrogenase (LDH) into the medium is a consequence of cellular integrity damage. Cells were seeded in a 96-well plate and treated with GO for 24 hours. The cells treated with LDH release solution were defined as positive control (P-ctr), while cells without treatment were defined as negative control (N-ctr), and the medium without cells was defined as blank control. The evaluation was based on a coupled enzymatic conversion from a tetrazolium salt into a formazan product, and the absorbance was measured at 490 nm by the Infinite M200 Microplate Spectrophotometer (Tecan). All values were normalized against the P-ctr (100% LDH leakage) and the blank control (0% LDH leakage).

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