The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA): anti-BRK (sc-916 and sc-1188), antiphosphotyrosine PY20 (sc-508), anti-SMAD2/3/4, antitubulin (sc-9104), anti-GFP (sc-8334), and anti–β-actin (sc-130300). Anti–α-tubulin mouse monoclonal (T9026) antibody was purchased from Sigma-Aldrich (St. Louis, MO). Anti-Halo rabbit polyclonal antibody (G9281) and Magne HaloTag magnetic affinity beads were purchased from Promega (Madison, WI). Proteasome inhibitor MG132 was obtained from Sigma-Aldrich (St. Louis, MO).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.