HeLa cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (FCS) (Thermo Fisher Scientific) at 37°C under 5% CO2. Monocytes were purified by centrifugation on Ficoll (Sigma-Aldrich) for 20 min at 800g, and mononucleated cells were subsequently passed through EasySep magnetic beads (STEMCELL Technology). Monocyte purity was verified by flow-cytometry by means of CD14 labeling. MDDCs were generated by cultivating monocytes in RPMI 1640 FCS medium supplemented with granulocyte-macrophage colony-stimulating factor at 100 ng/ml and interleukin-4 at 25 ng/ml for 6 days. Differentiation to MDDCs was verified by FACS based on CD14/CD209 labeling.

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