Negative-stain EM was performed with purified ADDomer (0.1 to 0.5 mg/ml) dialyzed into 25 mM Hepes (pH 7.5), 150 mM NaCl, and 2 mM EDTA. Sample was stained with 3% uranyl acetate. Micrographs were recorded using a 200-kV FEI Tecnai T20 microscope with an Eagle 4k × 4k charge-coupled device camera.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.