The PMMA resist analyte solution was produced by dissolving PMMA with an average weight of 350,000 g/mol in anisole with a concentration of 3 mg/ml. The thin film was deposited by spin-coating the PMMA solution (3 mg/ml) onto the metasurface using 6000-rpm spin speed. The same procedure was carried out for the IRRAS PMMA reference measurement.

For the bioassay measurements, polylysine was diluted in phosphate-buffered saline solution to a concentration of 100 μg/ml. The metasurface chip was incubated with the polylysine to allow polymer physisorption.

The single-stranded DNA aptamer molecules were prepared in 10 mM (pH 7.4) phosphate-buffered saline with 20 μM concentration and bound to the polylysine-coated chip surface. As an aptamer-specific binding molecule, human ODAM sample solution (0.25 mg/ml) was prepared by using the same buffer conditions as for DNA aptamer solution preparation. The used DNA sequence for the ODAM specific aptamer was as follows (28): 5′-CCATTCGTACGCAACAGGGATGCATCGACTGTAAAC ACGTGGATGGCTCTGAATGC-3′.

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