Image processing was done in ImageJ (adding scale bars and generating maximum projections) and Imaris. Nuclei stained with DAPI were counted to obtain the total number of cells per image. The PLA puncta were counted in a three-dimensional rendering of each Z-stack in Imaris using the Spot tool. The same Spot parameters (estimated puncta XY and Z diameter, threshold) were used for all samples in all experiments. The puncta count value for each Z-stack was divided by the total number of cells per image, and results were plotted in Prism 8 (GraphPad).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.