Image processing was done in ImageJ (adding scale bars and generating maximum projections) and Imaris. Nuclei stained with DAPI were counted to obtain the total number of cells per image. The PLA puncta were counted in a three-dimensional rendering of each Z-stack in Imaris using the Spot tool. The same Spot parameters (estimated puncta XY and Z diameter, threshold) were used for all samples in all experiments. The puncta count value for each Z-stack was divided by the total number of cells per image, and results were plotted in Prism 8 (GraphPad).

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