Deconvoluted PLA images were acquired with a DeltaVision Image Restoration Inverted Olympus IX-71 microscope using a 100× oil immersion objective. Excitation/emission wavelengths were 390/435 nm for the blue channel (DAPI) and 575/632 nm for the red channel (PLA puncta). Exposure times and transmittance percentages were held constant while imaging all samples within the same experiment. At least three Z-stack images (0.2-μm thickness per slice) of different fields of view were captured per coverslip for each control, and at least five Z-stack images were captured for all other samples. Images from maximum projection of Z-stack images and Imaris spot analysis result snapshots are shown in Fig. 5.

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