Deconvoluted PLA images were acquired with a DeltaVision Image Restoration Inverted Olympus IX-71 microscope using a 100× oil immersion objective. Excitation/emission wavelengths were 390/435 nm for the blue channel (DAPI) and 575/632 nm for the red channel (PLA puncta). Exposure times and transmittance percentages were held constant while imaging all samples within the same experiment. At least three Z-stack images (0.2-μm thickness per slice) of different fields of view were captured per coverslip for each control, and at least five Z-stack images were captured for all other samples. Images from maximum projection of Z-stack images and Imaris spot analysis result snapshots are shown in Fig. 5.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.