Information on the source of all Abs used in the SBA can be found in table S1. HPA Abs from the HPA are commercially available from Atlas Antibodies AB. HEK293 FreeStyle cells (HEK293F), FreeStyle 293 Expression media, FreeStyle MAX Reagent, and 125-ml culture flasks were from Thermo Fisher Scientific. PE-conjugated HA and FLAG Abs, as well as unconjugated HA Ab, were from BioLegend. 1D4 and OLLAS Abs were conjugated to PE using an Ab conjugation kit from Abcam (Cambridge, MA) according to the manufacturer’s protocols. Half-volume, 96-well plates were from Greiner (Tucson, AZ). Blocking reagent for ELISA (enzyme-linked immunosorbent assay) (BRE) and cOmplete protease inhibitor tablets were from Roche (Basel, Switzerland). Phosphate-buffered saline (PBS) was from Medicago. ProClin 300, casein, polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), and FLAG M2 Ab were from Sigma-Aldrich (St. Louis, MO). n-Dodecyl-β-d-maltoside (DM) was from Anatrace. Rabbit IgG was from Bethyl. Expression constructs of human GPR182 and GPR4 were obtained from cDNA.org. Anti-mouse IgG– and anti-rabbit IgG–conjugated R-PE were from Jackson ImmunoResearch.

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