CD spectra were collected using a Chirascan CD spectrometer at 23°C and a quartz cuvette with a 1-mm path length. Data were collected in the wavelength range of 190 to 260 nm, using a 1-nm bandwidth, 1-nm steps, and an averaging time of 0.5 s. Every measurement was repeated eight times, and data were then averaged and smoothed (5°) using built-in options of Chirascan Q100 software.

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