A gelatin stock solution was used to prepare the sacrificial ink for SWIFT. The 15% (w/w) gelatin stock solution was made by slowly adding 45 g of gelatin into 255 ml of PBS without calcium or magnesium at 85°C while stirring. A separate gelatin ink was created for printing tissue molds used in the perfusion chambers following the same protocol temperature, except that a temperature of 70°C was used. In each case, the samples were held at temperature and stirred for 12 hours to fully dissolve the gelatin, after which their pH was adjusted, while stirring, to ~7.5 using 2 N NaOH. Each hot gelatin stock solution was sterile-filtered, aliquotted, and stored at 4°C for up to 6 months.

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