Assembly and crystallization of NP superlattices
This protocol is extracted from research article:
Unusual packing of soft-shelled nanocubes
Sci Adv, May 17, 2019; DOI: 10.1126/sciadv.aaw2399

The assembly was obtained by mixing equal molar amounts of type A and type B DNA-functionalized Au NCs, and a linker Ln in a certain mole ratio of linker/type A (or B) DNA-functionalized NCs. The NCs were allowed to aggregate at room temperature. The samples were then annealed at a temperature ~3° to 5°C below the melting temperature of the assembled particles for a period of 30 min to several hours, depending on the sample. The annealed samples were transferred with buffer to a quartz capillary (1.0-mm diameter) and sealed with wax for SAXS measurements.

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