To generate the LocScale maps, unsharpened and unfiltered cryo-EM maps were scaled against simulated model maps using a rolling window of 20 voxels. The model maps were simulated from full-chain models refined against cryo-EM maps that were sharpened in RELION2. The dmin parameter of the LocScale procedure was set to the Nyquist frequency (2.7 Å), as recommended by the authors (32). Two rounds of LocScale were performed for the Elp123 lobe map, and one round was performed for the Elp123 lobe DD map.

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