A total of 1.0 × 105 of CD90 BM-MSCs were seeded in the lower chambers of a 24-well Transwell culture plate and cocultured at 37°C and 5% CO2 with an equal number of HEK293T cells that were transduced with either a control AAV vector or the AAV.COX-2 vector in the upper chambers. The MSCs in the lower chambers were harvested on days 1 and 6 for analysis of CD90 expression by FACS.

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