All data were presented as means ± SEM. Two-sample student’s t test, one-way analysis of variance (ANOVA), and Tukey post hoc test were used to compare differences among two or multiple groups, respectively. For two independent variables, two-way ANOVA and Bonferroni post hoc comparison were adopted. For comparison of g-ratios, Kruskal-Wallis test was adopted because of non-Gaussian distribution of sample data. A significant level was defined when P < 0.05. All statistical analysis was performed by GraphPad Prism 7.0 (La Jolla, CA, USA).

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