Experimental design
Western blotting
Immunofluorescence staining
Virus injection and in vivo two-photon calcium imaging
Dendritic spine imaging
Whole-cell patch-clamp recording
Electron microscopy
Statistical analysis
The motor learning ability was assessed by an accelerating rotarod (Ugo Basile, Comerio, Italy), which started at 5 rpm and sped up to 80 rpm within 5 min. Each mouse received three training sessions at 9:00 a.m., with each session on one of three consecutive days.