ABRs to pure tones were used to ensure normal cochlear function before toxicity studies and determine threshold shifts following kanamycin treatment (17). Briefly, needle electrodes were placed subcutaneously below the test ear, at the vertex, with a ground electrode near the paw. Each ear of each anesthetized mouse was stimulated individually with a closed tube sound delivery system sealed into the ear canal. The ABR to 1-ms rise-time tone burst stimuli at 4, 8, 12, 16, 24, 32, and 48 kHz was recorded, and thresholds were obtained for each ear. Only mice with normal bilateral, baseline ABR thresholds were used.

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