Transmission electron microscopy

Culture cells were fixed with 1% glutaraldehyde in phosphate-buffered saline (PBS) at pH 7.3 for 1 hour at 4°C and then post-fixed with 1% osmium tetroxide in 0.1 M phosphate buffer at pH 7.3 for 1 hour at 4°C and dehydrated in a graded series of ethanol. Following dehydration, they were embedded in Epon 812 (TAAB Laboratories Equipment, Berkshire, England) and then observed under a JEM-1400 Plus electron microscope (JEOL, Japan).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.