Transmission electron microscopy

Culture cells were fixed with 1% glutaraldehyde in phosphate-buffered saline (PBS) at pH 7.3 for 1 hour at 4°C and then post-fixed with 1% osmium tetroxide in 0.1 M phosphate buffer at pH 7.3 for 1 hour at 4°C and dehydrated in a graded series of ethanol. Following dehydration, they were embedded in Epon 812 (TAAB Laboratories Equipment, Berkshire, England) and then observed under a JEM-1400 Plus electron microscope (JEOL, Japan).

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