Splenic CD4+Foxp3CD45RBhi cells were sorted from Foxp3GFPCD45.1+ mice and mixed with Treg cells sorted from lymph nodes and spleens of Bcl11b+/+Foxp3IRES-YFP-Cre or Bcl11bfl/flFoxp3IRES-YFP-Cre mice. Colitis was induced in RAG1-deficient hosts by intravenous injection of 8 × 105 CD45RBhi + 2 × 105 Treg cells. Mice weight was recorded blindly once a week for 8 weeks.

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