ATAC-seq was performed as previously described (28, 36) on 50,000 sort-purified YFP+ Treg cells from Bcl11b/Treg KO and HT mosaic mice. Library quality and adapter dimer contamination were assessed on a Bioanalyzer (#5067-4626, Agilent). Libraries were sequenced as PE75 on a NextSeq500.

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