All work was compiled with the U.K. Home Office guiding principles for the care and use of laboratory animals. Mice carrying a conditional Pdx1-Cre KrasG12D/+ allele were used and have been described previously (26). Aging experiments were carried out on male WT C57BL/6 mice or male nfkb1−/− mice on a pure C57BL/6 background at 6.5, 9.5, and 24 months of age. For hydrodynamic tail vein injection experiments, tlr2−/− mice on a C57BL/6 background were purchased from the Jackson Laboratory (JAX). Male and female tlr2−/− mice and WT siblings, aged between 8 to 12 weeks, were included in the study. Plasmids for hydrodynamic injection were prepared using the Qiagen Plasmid Maxi Kit (Qiagen, Germany), as per the manufacturer’s instructions. Animals received 6 μg of a sleeping beauty transposase–encoding plasmid (CMV-SB 13 transposase), and 20 μg of NRasG12V/green fluorescent protein (GFP) encoding plasmid (pT3-NRasG12V-IRES-GFP) was diluted in physiological saline to 10% of the animal’s body weight (approximately 2 ml) and delivered via the lateral tail vein within 10 s. Plasmid encoding an NRasG12V effector loop mutant (pT3-NRasG12V/D38A-IRES-GFP), incapable of downstream NRas signaling, was used as a control. Mice were culled after 6 days, and liver tissue was harvested.

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