Cells on slides were fixed with 4% PFA for 15 min and washed with PBS. A 2% donkey serum blocking buffer in PBS was used for 1 hour at room temperature. Primary antibody incubation was done overnight. After PBS washes, secondary antibody was added for 1 hour at room temperature. After PBS washes, slides were mounted with mounting media containing DAPI (4′,6-diamidino-2-phenylindole). For the list of antibodies and concentrations, refer to table S2.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.