Cells on slides were fixed with 4% PFA for 15 min and washed with PBS. A 2% donkey serum blocking buffer in PBS was used for 1 hour at room temperature. Primary antibody incubation was done overnight. After PBS washes, secondary antibody was added for 1 hour at room temperature. After PBS washes, slides were mounted with mounting media containing DAPI (4′,6-diamidino-2-phenylindole). For the list of antibodies and concentrations, refer to table S2.

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