Genome assembly, gene prediction, and annotation
This protocol is extracted from research article:
Genetic basis of species-specific genitalia reveals role in species diversification
Sci Adv, Jun 26, 2019; DOI: 10.1126/sciadv.aav9939

The cleaned sequence reads of C. uenoi genome in three libraries (insert sizes: 170 bp, 2 kbp, and 5 kbp) were assembled using Platanus version 1.2.1 (49) with the default parameter settings. Methods of subsequent gene prediction and annotation with the assembled sequences are described in Supplementary Methods. The completeness of the predicted gene set and assembled genome was assessed using the BUSCO pipeline version 3.0.2 (20) with the Endopterygota single-copy orthologs (endopterygota_odb9).

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