Sections were incubated in freshly prepared COX medium [100 μM cytochrome c, 4 mM diaminobenzidine tetrahydrochloride, catalase (20 μg/ml), and 0.2 M phosphate buffer (pH 7.0)]. After incubation for 45 min at 37°C, slides were washed three times in PBS. Thereafter, slides were incubated in freshly prepared SDH medium [130 mM sodium succinate, 200 μM phenazinemethosulphate, 1 mM sodium azide, 1.5 mM nitroblue tetrazolium, and 0.2 M phosphate buffer (pH 7.0)] for 30 min for colon and quadriceps and 10 min for heart at 37°C. Last, slides were washed three times with PBS, dehydrated (following concentration of ethanol: 70%, 75%, 95%, and 2× 99.5%) and mounted for bright-field microscopy. The sections for laser capture dissection (14 μm) were similarly exposed to COX/SDH staining, dehydrated, and air-dried for 60 min.

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