Sections were incubated in freshly prepared COX medium [100 μM cytochrome c, 4 mM diaminobenzidine tetrahydrochloride, catalase (20 μg/ml), and 0.2 M phosphate buffer (pH 7.0)]. After incubation for 45 min at 37°C, slides were washed three times in PBS. Thereafter, slides were incubated in freshly prepared SDH medium [130 mM sodium succinate, 200 μM phenazinemethosulphate, 1 mM sodium azide, 1.5 mM nitroblue tetrazolium, and 0.2 M phosphate buffer (pH 7.0)] for 30 min for colon and quadriceps and 10 min for heart at 37°C. Last, slides were washed three times with PBS, dehydrated (following concentration of ethanol: 70%, 75%, 95%, and 2× 99.5%) and mounted for bright-field microscopy. The sections for laser capture dissection (14 μm) were similarly exposed to COX/SDH staining, dehydrated, and air-dried for 60 min.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.