Nb transgenic plants carrying 35S:βC1 have been reported previously (8). Nb, S. lycopersicum (Zhongza no.9; tomato), and Gossypium barbadense (Xinhai no. 21; cotton) plants were grown in a growth chamber at 25°C with a 12-hour light/12-hour dark cycle. G. hirsutum BD18 containing Bt gene was used as Bt/cotton plants.

A. thaliana ecotype Columbia (Col-0, At), βC1-1/At, and βC1-3/At (14), wrky20-1 (SALK_055904), wrky20-2 (SALK_116115), pdf1.2a (SALK_063966), myc2-1 (18), and myc234 (20) mutants were used in this study. We generated quadruple wrky20-2/myc234 mutants by crossing the corresponding parental single wrky20-2 and triple myc234 homozygous lines. After vernalization for 2 days at 4°C in the dark, sterilized seeds on Murashige and Skoog (MS) medium were transferred to a growth chamber at 22°C with a 12-hour light/12-hour dark cycle.

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