Mouse colons and small intestinal segments were swiss-rolled and fixed in 10% formalin, followed by paraffin embedding. Tissue sections (4 μm) were stained with hematoxylin and eosin for histological analysis using standard procedures.

For immunohistochemistry, organs were immediately embedded in O.C.T. (Tissue-Tek) cryosection medium and shock frozen on dry ice. Sections (7 μm) were cut, and samples were fixed with fresh acetone for 1 min, treated with anti-CD16/32 Fc-block, stained with appropriate antibodies, and detected with Alexa Fluor 568– and FITC-conjugated secondary antibodies. mTmG cryosections were directly analyzed without further processing. All sections were embedded in 4′,6-diamidino-2-phenylindole containing Fluoroshield (Sigma-Aldrich) and analyzed on an Axio Observer Z1 (Zeiss) microscope. Fiji software (37) was used for image processing.

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