The SKOV3 tumors were established in BALB/c female nude mice as described above. The tumors were inspected by visual observation and palpation. When the tumor volume reached approximately 20 mm3, the mice were randomly divided into three groups (n = 4): (i) vehicle (PBS) only, (ii) pacRNANClv, and (iii) pacRNAClv. Samples were injected via the tail vein once every fourth day for 28 days. The volume of tumors and weight of mice were recorded before every treatment and the fourth day after the last treatment. Antitumor activity was evaluated in terms of tumor size (V = 0.5ab2; a, long diameter; b, short diameter) by measuring two orthogonal diameters at various time points.

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