Each session consisted of one finger somatotopic mapping run of 9.9-min duration and two or three prediction task runs of 12-min duration. No participant was in the scanner for longer than 120 min per session.

Here, the same fMRI sequence and image reconstruction pipeline were used as in previously published work (16). In short, slice-selective slab-inversion VASO (38) was used on a 7T scanner (Siemens Healthineers, Erlangen, Germany), equipped with a 32-channel radiofrequency (RF) coil (Nova Medical, Wilmington, MA, USA) and a SC72 body gradient coil. Third-order B0 shimming was performed with three iterations. The timing of the acquisitions was TI1/TI2/TR = 1100/2845/3490 ms. The coil-combined data consist of interleaved BOLD and VASO contrasts, obtained as separate yet simultaneous time series. These time series are corrected for rigid volume motion and are separated by contrast with the effective temporal resolution of TR = 3.49 s for each individual contrast. The nominal resolution was 0.71 mm across cortical depths with 1.8-mm-thick slices perpendicular to the postcentral bank of the right central sulcus. VASO contrast was corrected for BOLD contaminations by the division of blood-nulled MR signal and not-nulled MR signal across consecutive TRs [for details, see (42)]. Imaging slice position and slice angle were adjusted individually for every participant to be perpendicular to the finger region of S1. This was performed on the basis of one to four short EPI test runs with five measurements (approximately 22 s per test scan) and their online depiction in the vendor-provided three-dimensional (3D) viewer.

If time permitted, slab-selective high-resolution (0.437 mm × 0.438 mm × 1.2 mm) anatomical data were collected covering the right S1 with MP2RAGE (Fig. 1A) (43). Those anatomical data were not used in the pipeline for generating cortical profiles. They were used to compare the approximate position of the cytoarchitectonically defined cortical layers to the 11 reconstructed cortical depths, in which the data were processed.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.