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In each farm, core microbes were corrected for diet. Thereafter, the samples in the farm were partitioned into two groups, winter (fall equinox to spring equinox) and summer (spring equinox to fall equinox). Then, each microbial OTU abundance was compared using Wilcoxon rank-sum test that was used to test for the difference between the abundance of the given OTU between the two seasons, followed by a multiple comparison correction using the Bonferroni method. Core microbial OTUs with corrected P < 0.05 in at least one farm were considered as showing a seasonal association.

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