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The marker gene sequences coming from each domain’s primer set (Archaea, Bacteria, Prokaryote, Ciliate, protozoa, and Fungi) were clustered using the 97% nucleotide sequence similarity threshold, using the UCLUST algorithm (43), following the QIIME command: pick_otus.py -m uclust -s 0.97). Representative OTUs for each OTU cluster were chosen with QIIME command pick_rep_set.py -m most_abundant.

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