DMEM + 10% FBS was used to make all grids; when the term “medium” is used, it should be assumed that serum is present. Typically, 1 ml of medium was pipetted into the dish so that the medium completely covered the bottom, the dish was tilted, ~0.9 ml of medium was retrieved and discarded, the thin film of medium was left behind on the bottom of the dish overlaid with 3 ml of FC40, and a grid or individual chambers were made using the printer essentially as described previously (9).

The reference plate used in Fig. 4 is a custom-made product supplied by iotaSciences; it consists of a glass support with a series of unique identifiers printed on it in a 16 × 16 matrix (i.e., A1 to A16 on the first row and P1 to P16 on the last row; 1.9-mm spacing between identifiers).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.