DMEM + 10% FBS was used to make all grids; when the term “medium” is used, it should be assumed that serum is present. Typically, 1 ml of medium was pipetted into the dish so that the medium completely covered the bottom, the dish was tilted, ~0.9 ml of medium was retrieved and discarded, the thin film of medium was left behind on the bottom of the dish overlaid with 3 ml of FC40, and a grid or individual chambers were made using the printer essentially as described previously (9).

The reference plate used in Fig. 4 is a custom-made product supplied by iotaSciences; it consists of a glass support with a series of unique identifiers printed on it in a 16 × 16 matrix (i.e., A1 to A16 on the first row and P1 to P16 on the last row; 1.9-mm spacing between identifiers).

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