Statistical analyses were performed using Excel (Microsoft) and GraphPad Prism 6.0 software. Student’s t test and one-way analysis of variance (ANOVA) were used to determine the statistical significance. P < 0.05 was considered significant. All replicate numbers (number of mice, number of spectrin rings, or number of OHCs and IHCs analyzed) are specifically indicated in the figure legends. When possible, all analyses were undertaken blind to genotype and/or development stage.

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