The QE mass spectrometer (Thermo Fisher Scientific) was outfitted with a heated electrospray ionization probe (HESI) with the following parameters: evaporation temperature, 120°C; sheath gas, 30; auxiliary gas, 10; sweep gas, 3; and spray voltage, 3.6 kV for positive mode and 2.5 kV for negative mode. Capillary temperature was set at 320°C, and S-lens was 55. A full scan range was set at 60 to 900 mass/charge ratio (m/z) with the resolution set to 70,000. The maximum injection time was 200 ms. Automated gain control was targeted at 3 million ions.

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