Flow cytometry was performed to measure the percentage of CXCR4+ UM cells. Cultured human UM cells were dissociated with a non-enzymatic cell dissociation solution (Sigma-Aldrich, St. Louis, MO), washed, and immunolabeled for 20 min at 4°C with an allophycocyanin (APC) mouse anti-human CD184 antibody (CXCR4 is also known as CD184, clone 12G5) (BD Biosciences, San Jose, CA). Data acquisition was performed using a BD FACSAria IIu cell sorter (BD Biosciences, San Jose, CA). FlowJo software (Tree Star, Ashland, OR) was used for data analysis.

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