P. xuthus larvae were staged from the third molting period, and fenoxycarb was applied during the JH-sensitive period. Fenoxycarb was a gift from H. Kataoka (The University of Tokyo), and it was dissolved in acetone (Wako Pure Chemical Industries, Ltd., Japan) to a final concentration of 1 μg/μl. A total of 5 μg of fenoxycarb was applied on the dorsal side of the larval integument, and 5 μl of acetone was used as a negative control. The larvae treated with fenoxycarb or acetone were reared separately.

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