IHC and quantitative image analysis were performed on 5-μm tissue sections mounted on glass slides, as previously described (41). Antibodies used in this study were mouse anti-CD68 (1:400; clone KP1, Dako), mouse anti-CD163 (1:400; clone 10D6, Novocastra/Leica), rabbit monoclonal anti-CD4 (1:200; clone EPR6855, Abcam Inc.), rabbit anti-APOBEC3G (1:500; HPA001812, Sigma), rabbit anti–granzyme B (1:200; HPA003418, Sigma), mouse anti-Mx1 (1:2000; clone M143; a gift from G. Kochs and the Department of Virology of the University of Freiburg), rabbit anti-Mx2 (1:500; HPA030235, Sigma), rabbit anti-Tetherin/BST2 (1:1000; HPA017060, Sigma), mouse anti-TNFα (1:1000; clone P/T2, ab9579, Abcam), and rabbit anti-TRIM5α (1:1000; HPA023422, Sigma).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.