Cell proliferation assays were performed using the MTT assay (M6494, Thermo Fisher Scientific) according to the manufacturer’s instructions. For cell cycle analysis, cells treated with compounds and vehicles reported in the figures and fixed in cold 70% ethanol were analyzed, as previously described (36), using a BD FACSVerse (BD Biosciences). Apoptosis was determined with an APC Annexin V apoptosis detection kit with PI (640932, BioLegend) according to the manufacturer’s protocol. Results shown were obtained from three independent experiments. See the Supplementary Materials and Methods for details.

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