The following antibodies were used for immunoprecipitation and Western blot analyses: C-terminal anti-ERα (sc-543, Santa Cruz Biotechnology), rabbit polyclonal anti-DOT1L (A300-953A, Bethyl Laboratories), β-actin (A1978, Sigma-Aldrich), N-terminal anti-ERα (ab75635), mouse anti-KMT4/DOT1L (ab72454), anti-histone H3 (total; ab1791), anti-H3K79me1 (ab2886), anti-H3k79me2 (ab3594), anti-H3K79me3 (ab2621), anti-H3K4me3 (ab7766), anti-H3K9me3 (ab1220), anti-H3K27me3 (ab24684) from Abcam, anti–cleaved caspase-3 (2305-PC-100, Trevigen), anti-TAP (CAB1001), and rabbit immunoglobulin G (IgG) isotype control (31235) from Thermo Fisher Scientific.

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