The following primary antibodies were used for Western blotting and immunostaining in this study: mouse anti-Flag (Sigma-Aldrich, F3165), rabbit anti-Flag (Proteintech, 20543-1-AP), rabbit anti-Vps4B (Proteintech, 17673-1-AP), mouse anti-P62 (Abcam, ab56416), rabbit anti-LC3B (Abcam, ab48394), mouse anti-V5 (Proteintech, 66007-1-Ig), mouse anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech, 60004-1-Ig), mouse anti–β-tubulin class III (Tuj 1; Abcam, ab78078), rabbit anti–Tuj 1 (Sigma-Aldrich, T2200), and mouse anti–β-actin (Cell Signaling Technology, 3700). The horseradish peroxidase–conjugated secondary antibodies were as follows: goat anti-mouse (Sigma-Aldrich, A4416) and goat anti-rabbit (Sigma-Aldrich, A9169). The fluorophore-conjugated secondary antibodies were as follows: goat anti-rabbit Alexa Fluor 488 (Life Technologies, A11036), goat anti-mouse Alexa Fluor 488 (Life Technologies, A11029), goat anti-mouse Alexa Fluor 568 (Life Technologies, A11031), goat anti-rabbit Cy5 (Life Technologies, A10523), and goat anti-mouse Cy5 (Life Technologies, A105234).

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