Circular dichroism spectra (195 to 260 nm) were collected on a J-815 spectropolarimeter (JASCO Inc., Japan) to evaluate the secondary structure of the peptides and their complexes (63, 64), individual peptides or complexes dissolved in PBS with the final concentration at 10 μM. Thermal denaturation of peptide complexes was monitored from 20° to 100°C at 222 nm with a thermal gradient of 5°C/min. The midpoint of the Tm values was acquired by JASCO software utilities.

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