Cells were fixed and incubated with primary antibodies at a dilution of 1:100, fluorescence dye–conjugated secondary antibodies, and 4′,6-diamidino-2-phenylindole (DAPI), according to standard protocols. Immunofluorescent microscopic images of the cells were obtained and viewed with an IX81 confocal microscope system (Olympus America).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.