Rabbit polyclonal antibodies against KHK-A and KHK-C and KHK-A pS80 and p62 pS28 antibodies were obtained from Signalway Biotechnology (Pearland, TX). The antibody generation procedures were described previously (32). Normal mouse immunoglobulin G (IgG) (sc-2025), TRIM21 (sc-25351), GST (sc-138), Nrf2 (sc-13032), tubulin (sc-8035), ERK1/2 (sc-514302), pERK1/2 (Thr202/Tyr204) (sc-16982), PRPS1/2 (sc-100822), and pan–KHK-A/C (sc-377411) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit antibodies against mitogen-activated protein kinase-activated protein kinase 2 (MAPK/APK2) (#3042), MAPK/APK2 pT222 (#3316), c-Jun (#9165), c-Jun pS73 (#9164), Trx1 (#2429), AMPKα (#5831), AMPKα pT172 (#2535), ACC1 (#3676), ACC1 pS79 (#11818), LC3B (#3868), cleaved PARP (Asp214) (#9541), and anti-rabbit IgG conformation-specific secondary antibody (L27A9) (#3678) were purchased from Cell Signaling Technology (Danvers, MA). BSO (B2515), NAC (A7250), Trolox (238813), MG132 (M7449), mouse monoclonal anti-Flag (F1804), rabbit anti-Flag (F7425), and anti-His (SAB1305538) antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Anti-Flag M2 agarose beads and [γ-32P]ATP were purchased from MP Biochemicals (Santa Ana, CA). Active AMPK proteins (#P47-10H) were obtained from SignalChem (Richmond, BC, Canada). A769662 was purchased from Tocris Bioscience (Avonmouth, Bristol, UK). Rabbit anti-Ki67 antibody (#AB9260) and compound C were obtained from Millipore (Billerica, MA). Anti-Keap1 (ab226997) and anti-p62 (ab56416) antibodies were obtained from Abcam (Cambridge, MA). Ub-K63 antibody (HWA4C4) was purchased from Novus (Littleton, CO). Anti–proliferating cell nuclear antigen (PCNA) (#610665) and hypoxia-inducible factor 1α (#610958) antibodies were obtained from BD Biosciences (Bedford, MA). An anti-PRPS1 T225 phosphorylation antibody was generated as previously described (32). U0126, SP600125, and SB203580 were purchased from EMD Biosciences (San Diego, CA). A fluorescein isothiocyanate (FITC)–labeled antibody against 5-bromo-2′-deoxyuridine (BrdU) (BU20A, 11-5071) was purchased from eBioscience.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.