Genomic DNA of mouse 10T½ fibroblasts, mouse C2C12 myoblasts, human HEK 293 cells, and human iPSCs was isolated using DirectPCR (cell) lysis reagent (VIAGEN) according to the manufacturer’s protocol. Genomic DNA of mouse muscle tissues was isolated using GeneJET genomic DNA purification kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. Genomic DNA was PCR amplified using GoTaq DNA polymerase (Promega) or with primers. PCR products were gel purified and subcloned into pCRII-TOPO vector (InvitroGen) according to the manufacturer’s protocol. Individual clones were picked, and the DNA was sequenced. Primer sequences are listed in table S1.

Mismatched duplex DNA was obtained by denaturing/renaturing of 25 μl of the genomic PCR product using the following conditions: 95°C for 5 min, 95° to 85°C (−2.0°C/s), 85° to 25°C (−0.1°C/s), hold at 4°C. Then, 25 μl of the mismatched duplex DNA was incubated with 2.7 μl of 10× NEB buffer 2 and 0.3 μl of T7E1 (New England BioLabs) at 37°C for 90 min. The T7E1-digested PCR product was analyzed by 2% agarose gel electrophoresis.

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