Bacteria were grown overnight at 37°C. Each bacterial suspension (2 μl) was spotted onto the respective bacterial nutrient agar and allowed to dry. Once dried, 10 μg of CadA, an equivalent volume of mock-purified CadA (from E. coli with and empty expression plasmid), or 10 μg of BSA was spotted adjacent to but not touching the bacterial colonies and allowed to dry. After drying completely, the plates were placed in a 37°C incubator and imaged every day for 4 days.

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