The KillerRed system was a gift from L. Lan (University of Pittsburgh School of Medicine, PA). Activation of KillerRed followed the method as previously described (62). Briefly, U2OS tet response element (TRE) cells with transfection of pBROAD3/tetR-KR were pretreated without or with 0.1 μM COH34 for 1 hour before exposure to a 15-W white fluorescent bulb for 10 min (height to light is 15 cm). Following 1 or 10 min of recovery, samples were examined by immunofluorescence with anti-KillerRed antibody (Evrogen), anti-PAR monoclonal antibody (Trevigen), or anti-XRCC1 antibody (GeneTex). Images were taken from a fluorescence microscope and analyzed by ImageJ.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.