Glucose uptake was carried out in primary adipocytes and L6 myotubes, as previously described (20). Briefly, cells were deprived of serum and then treated with or without insulin for 30 min. Glucose uptake was carried out in Hepes-buffered saline buffer containing 2-deoxy-d-[1,2–3 H(N)]glucose in the presence or absence of insulin for 10 min. Radioisotopes in cell lysates were measured using the Tri-Carb 2800TR scintillation counter for calculation of glucose uptake rates.

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