Liver TG was measured through determination of its glycerol contents, as previously described (42). Briefly, saponification of frozen liver chunks was carried out in ethanolic KOH to release free glycerol that was measured using the Free Glycerol Reagent (catalog no. F6428, Sigma-Aldrich). For liver TC measurement, lipids were first extracted using an organic solvent consisting of chloroform:isopropanol:NP-40 (7:11:0.1). After removal of the organic solvent, TC in the extracts was then determined using the LabAssay Cholesterol kit (catalog no. 294-65801, Wako Chemicals, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.