Telomeric, major satellite, and TERRA probes were labeled with using the T4 Polynucleotide Kinase kit. A total of 50 pmol of DNA was labeled with γ [32P ATP] by the PNK enzyme for 1 hour at 37°C.

Actin probes were labeled by using the Prime-a-Gene Promega kit. A total of 50 pmol of DNA was labeled with α [32P CTP] by the DNA polymerase I for 1 hour at room temperature. Probes were purified on microspin G25 columns (GE Healthcare no. 27-5325-01).

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