The PICh experiment procedure was adapted for telomeric regions using 0.25 μM telomere desthiobiotin-TEG-4XC18 spacers, 5′TtAgGgTtAgGgTtAgGgTtAgGgt-3′–specific locked nucleic acid (LNA) probes, and ≈8 × 108 mESCs following the previously described protocol (23). qPICh was performed, as previously described (21). For PICh/Western blot, 6 mg of chromatin was piched for each condition, hybridized with 12.5 μl of 100 μM telomeric probes, and captured with 500 μl of C1 MyOne beads. Eluted PICh material was resuspended in 60 μl of reverse cross-link solution, and 5 to 12 μl of PICh samples were processed for Western blotting.

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