The cold acclimation tests were performed on more than eight plates on at least three independent days. All error bars in the figures indicate the SEM. Assuming that the distributions of all data follow normal distribution, all statistical analyses were performed using parametric tests, the Tukey-Kramer method, Dunnett’s test, or the unpaired t test (Welch). Multiple comparisons were performed using one-way analysis of variance (ANOVA) with comparison tested using the Tukey-Kramer method and Dunnett’s test. Dunnett’s test was performed to compare the leftmost groups of the bar graphs with other groups. Comparisons between two groups were performed using the unpaired t test (Welch). *P < 0.05, **P < 0.01. We have performed two-way ANOVA in Fig. 2L (see “Supplementary raw data file”). The tests were performed using Mac statistical analysis version 2 (Esumi, Japan).

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